DNA Lab

     In science class, we just finished learning about biochemicals. DNA is an important biochemical in living organisms. Isolating DNA is one of the most basic, and essential techniques in the field of biology.  Extracting DNA allows scientists to detect genetic disorders, and  produce DNA fingerprints of individuals.  It also helps in the production of beneficial products such as insulin, antibiotics, and hormones.  Our fist step to perform this lab, was to gather our materials; a blender, peas, detergent,  distilled water, measuring cups, meat tenderizer, alcohol, test tubes, stirring rods, salt, and beakers. After we gathered our materials, we created two solutions.

     Our next step was to place the peas in the blender. The reason that the peas were placed in the blender was to extract DNA, and to break the cells open. A blender is a good tool for this. After they were blended well, the salt and detergent (solution one) were added. This solution breaks down the fat and protein that makes up the phospholipid bilayer of the pea cells. Then we strained the mixture, with a coffee filter and strainer.  Our next step was to measure out 25 mL of the meat tenderizer and distilled water solution (second solution) and add it to the mixture.  Finally we added the ethanol which caused the DNA to precipitate.

                                   

-Emma V.